Recombinant Human 4-1BB Ligand Protein-T&L Biotechnology

Product manual COA

Recombinant Human 4-1BB Ligand Protein

Price：$ 423/

Price：$ 701

Size：

50μg

50μg

100μg

Product No.：GMP-TL695-0050

Product No.：100μg / GMP-TL695-0100

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Product Information

Expression Host：
HEK293 cells
Synonym：
4-1BB Ligand, TNFSF9, CD137L
Protein sequence：
A DNA sequence encoding the human 4-1BB Ligand (NP_003802.1) was expressed with a Fc-tag at the C-terminus.
Molecular Mass：
The recombinant human 4-1BB Ligand protein consists of 467 amino acids and predicts a molecular mass of 51 kD.
QC Testing Purity：
> 90 % as determined by SDS-PAGE.
Endotoxin：
< 0.1EU per 1 μg of the protein by the LAL method.
Activity：
Measured by its ability to induce IL-8 secretion by PBMC. The ED₅₀ for this effect is ≤ 10 ng/mL.
Formulation：
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability：
24 months at 2℃ to 8℃ in lyophilized state.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage：
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
Avoid repeated freeze-thaw cycles.

Background

4-1BB ligand (4-1BBL), also known as CD137L or TNFSF9, is a member of the tumor necrosis factor (TNF) family expressed on activated antigen-presenting cells. Its receptor, 4-1BB, is a member of the TNF receptor family expressed on activated CD4 and CD8 T cells. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4⁺and CD8⁺T cells. T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response. Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

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