Recombinant Human IL-3 Protein-T&L Biotechnology

Product manual COA

Recombinant Human IL-3 Protein

Price：$ 352

Price：$ 507

Size：

50μg

50μg

100μg

Product No.：GMP-TL511-0050

Product No.：GMP-TL511-0100

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Product Information

Expression Host：
HEK293 cells
Synonym：
MCGF(Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
Protein sequence：
A DNA sequence encoding the human IL-3 (GenBank: AAA59146.1) was expressed with a His-tag at the C-terminus.
Molecular Mass：
The recombinant human IL-3 protein consists of 139 amino acids and predicts a molecular mass of 15.9 kD.
QC Testing Purity：
> 90 % as determined by SDS-PAGE.
Endotoxin：
< 0.1EU per 1 μg of the protein by the LAL method.
Activity：
Measured in a cell proliferation assay using TF‑1 cells, activity≥2.0×10⁵IU/mg.
Formulation：
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability：
Lyophilized preparation can be stored at -20℃.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage：
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
Avoid repeated freeze-thaw cycles.

Background

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of megakaryocytes, granulocyte-macrophages, erythrocytes, eosinophils, basophils, and progenitors of the mast cell line. IL-3 is produced by T cells, mast cells, and eosinophils and enhances platelet production, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunity epidemic regulation. Much of the activity of IL-3 depends on costimulation with other cytokines. IL-3 is a species-specific, mutable glycosylated cytokine.

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