Recombinant Human IL-12 Protein-T&L Biotechnology

Product manual COA

Recombinant Human IL-12 Protein

Price：$ 462

Price：$ 732

Price：$ 1127

Size：

50μg

50μg

100μg

1mg

Product No.：GMP-TL508-0050

Product No.：GMP-TL508-0100

Product No.：GMP-TL508-1000 (Customized)

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Product Information

Expression Host：
HEK293 cells
Synonym：
IL12, p70, Interleukin-12
Protein sequence：
Recombinant human IL-12 is heterodimeric and composed of P29459-1 (IL12A) and P29460-1 (IL12B).
Molecular Mass：
The Recombinant Human IL-12 Protein predicts a molecular mass of 65 kDa.
QC Testing Purity：
> 90 % as determined by SDS-PAGE.
Endotoxin：
< 0.1EU per 1 μg of the protein by the LAL method.
Activity：
Measured by cell proliferation assay on PHA activated PBMC cells.The ED50 of this effect is ≤ 0.2 ng/mL.
Formulation：
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability：
24 months at 2℃ to 8℃ in lyophilized state.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage：
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.

Background

IL-12 is primarily produced by monocytes, macrophages, B cells and DCs. IL-12 is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40).IL-12 receptors are located mainly on T cells and NK cells, and IL-12 can stimulate Th1 and NK cell growth, while inhibiting Th2 cell responses. IL-12 augments cytotoxicity of T lymphocytes and NK cells through the enhancement of IFN-γ and TNF-α secretion, and inhibition of IL-4 production.

References

Click to view the citations of recombinant human IL-12