Both methods are feasible

There will be no problem within a week

PBMCs that have been cryopreserved and revived can be used to culture NK cells, but the culture effect is not as good as fresh PBMCs

seven days.

DPBS or serum-free culture medium can be used. It is not recommended to contain calcium and magnesium ions in the buffer system.

unit/mg=10⁶/ED₅₀（ng/ml）

Purity is defined as the proportion of collected target cells and can be analyzed by flow cytometry. The seperation efficiency is defined as the proportion of recovered target cells relative to the target cells in the original sample.

The purity varies depending on the cell viability/status/T cell ratio of the sample.

Customers  conduct virus transfection experiments at 24h，48h or 72h, with 48h being the most common.  When the virus is transfected, magnetic beads can be retained.

Not recommended for reuse.

Can not, It suggests removing the magnetic beads first before testing the cells

magnetic beads need to be removed,general magnetic beads and magnetic frame can meet the application.

50nm.Magnetic beads need to go through seperation columns (It is compatible with Miltenyi MACS columns), which are biodegradable.

The naked beads of micrometer magnetic beads (GMP-TL603) and nano magnetic beads (TL-622, TL-623, TL-624) have completed safety evaluation and verification at the animal level.