Recombinant Human IL-6 Protein-T&L Biotechnology

Product manual COA

Recombinant Human IL-6 Protein

Price：$ 352

Price：$ 507

Size：

50μg

50μg

100μg

Product No.：GMP-TL512-0050

Product No.：GMP-TL512-0100

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Product Information

Expression Host：
HEK293 cells
Synonym：
IL6,Interleukin-6,BSF2,HSF,IFNB2
Protein sequence：
A DNA sequence encoding the human IL-6（NP_000591.1：V30-M212）was expressed with a His-tag at the C-terminus.
Molecular Mass：
The Recombinant Human IL-6 Protein predicts a molecular mass of 21.6 kD.
QC Testing Purity：
> 90 % as determined by SDS-PAGE.
Endotoxin：
< 0.1EU per μg of the protein as determined by the LAL method.
Activity：
Determined by the dose-dependent stimulation of the proliferation of TF-1 cells，the activity is ≥2.0x10⁷U/mg
Formulation：
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability：
24 months at 2℃ to 8℃ in lyophilized state.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
Avoid repeated freeze-thaw cycles.

Background

Interleukin 6 (IL-6) produced by fibroblasts, monocytes, macrophages, T lymphocytes, B lymphocytes, epithelial cells, keratinocytes and various tumor cells. IL-1, TNF-α, PDGF, viral infection, double stranded RNA, and cAMP can all induce normal cells produce IL-6. IL-6 can stimulate the proliferation, differentiation of immune responses and improve immune cell functions. IL-6R is widely expressed on the surface of activated B cells, quiescent T cells, NK cells, myeloma cells, hepatocytes, and myeloid leukemia cells. The main role of IL-6 is to promote the proliferation and differentiation of B cells and the secretion of antibodies. It also has extensive effects on liver cells, T cells, neural tissue, and hematopoietic system. IL-6 also has anti-tumor effects and can directly or indirectly enhance the tumoricidal activity of NK cells and CTL.

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