Recombinant Human IL-2 Protein ( high-efficiency )-T&L Biotechnology

Product manual COA

Recombinant Human IL-2 Protein ( high-efficiency )

Price：$ 42

Size：

100μg

100μg

Product No.：GMP-TL906-0100

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Product Information

Generic Name：
Recombinant Human IL-2 Protein
Expression Host：
E.coli
Synonym：
TCGF, lymphokine, Interleukin 2
Molecular Mass：
The recombinant human IL-2 protein predicts a molecular mass of 15.4 kD.
QC Testing Purity：
> 90 % as determined by SDS-PAGE.
Endotoxin：
< 0.1EU per 1 μg of the protein by the LAL method.
Activity：
Determined by the dose-dependent stimulation of the proliferation of CTLL-2 cells ,corresponding to a activity of ≥1x10⁷U/mg.
Formulation：
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability：
Lyophilized preparation can be stored at 2-8 ℃.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
Avoid repeated freeze-thaw cycles.

Background

Activated T cells generally cannot survive for a long time in vitro culture, and the addition of IL-2 can promote their long-term and sustained proliferation. The surface of stationary T cells does not express IL-2R and does not respond to IL-2; T cells activated by mitogen or other stimuli can express IL-2R and become target cells of IL-2; IL-2 can also induce increased IL-2R expression in target cells. The expression of IL-2R on T cells is transient, generally reaching its peak 2-3 days after activation and disappearing around 6-10 days. With the disappearance of IL-2R, T cells lose their ability to respond to IL-2. Therefore, in order to maintain the long-term growth of normal T cells in vitro, it is necessary to constantly stimulate T cells with mitogen or other stimulators to maintain the expression of IL-2R. NK cells are the only lymphoid cells that normally express IL-2R, and therefore always maintain reactivity to IL-2. However, only IL-2R is expressed on stationary NK cells β Chain and γ Chains have a low affinity for IL-2 and can only react with high concentrations of IL-2. Once NK cells are activated, they express IL-2R α Chain, becoming a high affinity receptor.

References

Click to view the cited literature of recombinant human IL-2