Recombinant Human IL-18 Protein-T&L Biotechnology

Product manual COA

Recombinant Human IL-18 Protein

Price：$ 211

Price：$ 394

Size：

50μg

50μg

1mg

Product No.：GMP-TL902-0050

Product No.：GMP-TL902-1000(Customized)

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Product Information

Expression Host：
E.coli
Synonym：
interleukin-18, Interleukin-1 gamma, IL1F4
Protein sequence：
A DNA sequence encoding the human IL-18 (NP_001553.1) was expressed with no tag.
Molecular Mass：
Predicts a molecular mass of 18.2 kD.
QC Testing Purity：
> 90 % as determined by SDS-PAGE
Endotoxin：
< 0.1EU per 1 μg of the protein by the LAL method.
Activity：
Measured by its ability to induce IFN-γ secretion by KG‑1 cells in the presence of TNF-α. The ED₅₀ for this effect is 5-30 ng/mL.
Formulation：
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability：
Lyophilized preparation can be stored at -20 ℃.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage：
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL. Avoid repeated freeze-thaw cycles.

Background

IL-18 is a proinflammatory cytokine that plays an important role in NK cell activation and Th1 cell response. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, γδ T cells, and phagocytes. IL-18 is related to the IL-1 family in terms of both structure and function. In terms of structure, IL-18 and IL-1beta share significant primary amino acid sequences and are similarly folded as all-beta pleated sheet molecules. Also similar to IL-1beta, IL-18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide. In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4⁺T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis.

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