Recombinant Human IL-21 Protein-T&L Biotechnology

Product manual COA

Recombinant Human IL-21 Protein

Price：$ 437

Price：$ 704

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Size：

50μg

50μg

100μg

1mg

Product No.：GMP-TL509-0050

Product No.：GMP-TL509-0100

Product No.：GMP-TL509-1000(Customized)

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Product Information

DMF filed no：
039117
Expression Host：
CHO cells
Synonym：
CVID11, IL-21, Za11
Protein sequence：
A DNA sequence encoding the extracellular domain of human IL-21(Q9HBE4-1: Q29-S162) was expressed with the C-terminal fused Fc region of human IgG1.
Molecular Mass：
The recombinant human IL-21 predicts a molecular mass of 41.9kD.
QC Testing Purity：
> 95 % as determined by SDS-PAGE.
Endotoxin：
< 0.01 EU per μg of the protein as determined by the LAL method.
Activity：
Measured by its ability of inducing human natural killer lymphoma NK-92 cells to secrete interferon-γ. The expected ED₅₀ is ≤ 50 ng/mL.
Formulation：
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability：
Lyophilized preparation can be stored at -20 ℃.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage：
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
Avoid repeated freeze-thaw cycles.

Background

IL-21 is a pleiotropic cytokine produced by CD4⁺ T cells in response to antigenic stimulation.Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include:inducing the differentiation of T-cell-stimulated B-cells into plasma cells and memory B-cells; the stimulation of IgG production in conjunction with IL-4;and the induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally,IL-21 promotes the anti-tumor activity of CD8⁺ T-cells and NK cells.IL-21 exerts its effect through binding to a specific type I cytokine receptor,IL-21R,which also contains the γ chain (γc) found in other cytokine receptors, including IL-2,IL-4,IL-7,IL-9 and IL-15.The IL-21/IL-21R interaction triggers a cascade of events,which includes activation of the tyrosine kinases JAK1 and JAK3,followed by activation of the transcription factors STAT1 and STAT3.

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