Anti-Human CD28 mAb-T&L Biotechnology

Product manual COA

Anti-Human CD28 mAb

Price：$ 141

Size：

500μg

500μg

Product No.：GMP-TL102-0500

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Product Information

DMF filed no：
038820
Expression Host：
Mouse Hybridoma cells
QC Testing Purity：
> 95 % as determined by SDS-PAGE
Purification：
Protein A purified from cell culture supernatant
Biological activity：
The binding rate with Jurkat cells shall not be less than 90%.
Endotoxin：
< 0.01EU per μg of the protein as determined by the LAL method
Formulation：
Supplied as a 0.22μm filtered solution in PBS, PH 7.4.
Stability & Storage
24 months at 2℃ to 8℃. Avoid repeated freeze-thaw cycles.
Applications
Soluble method: the concentration of 500 ng/mL is recommended, under which T cells can be activated and CD3⁺CD56⁺T cells can be expanded significantly.
Coating method: 10 mL PBS buffer (5μg/mL anti-human CD3) is placed in a T75 flask at 4°C overnight. Please remove the PBS and wash the flask with saline gently three times before use.

Background

The CD28 molecules express on the surface of most T cells and is considered to be unique to T cells. T cell reactivity to antigen is mainly mediated through the CD3 / TCR complex, but also depends on the synergy of other molecules. T cells can be efficiently activated by several pathways, including CD3 / TCR, CD2, and CD28. Mononuclear cells isolated from peripheral blood, bone marrow or cord blood can be the source of T cells proliferation in the presence of CD3, CD28 monoclonal antibodies and various other cytokines.

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