Anti-Human CD3 mAb-T&L Biotechnology

Product manual COA

Anti-Human CD3 mAb

Price：$ 141

Price：Please Inquire

Size：

500μg

500μg

1mg

5mg

Product No.：GMP-TL101

Product No.：GMP-TL101-1000(Customized)

Product No.：GMP-TL101-5000(Customized)

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Product Information

DMF filed no：
038125
Expression Host：
CHO cells
QC Testing Purity：
> 95 % as determined by SDS-PAGE.
Purification：
Protein A purified from cell culture supernatant.
Biological activity：
The binding rate with Jurkat cells shall not be less than 90%
Endotoxin ：
< 0.01EU per μg of the protein as determined by the LAL method.
Formulation：
Supplied as a 0.22μm filtered solution in PBS, PH 7.4.
Stability & Storage
24 months at 2℃ to 8℃. Avoid repeated freeze-thaw cycles.
Applications
Soluble method: the concentration of 500 ng/mL is recommended, under which T cells can be activated and CD3⁺CD56⁺T cells can be expanded significantly.
Coating method: 10 mL PBS buffer (5μg/mL anti-human CD3) is placed in a T75 flask at 4°C overnight. Please remove the PBS and wash the flask with saline gently three times before use.

Background

In immunology, CD3 (Differentiated Cluster 3) of T cells is a protein complex. CD3 has five peptide chains, namely, the γ, δ, ε, ζ, and η chains, all of which are transmembrane proteins. The transmembrane region of the CD3 molecule is connected to the transmembrane region of the two peptide chains of TCR through a salt bridge to form the TCR-CD3 complex, which jointly participates in the recognition of antigen. The activation signal generated by TCR antigen recognition is generated and transduced by CD3. Mononuclear cells isolated from peripheral blood, bone marrow, or umbilical cord blood can obtain large numbers of proliferating T lymphocytes in the presence of CD3 monoclonal antibodies and various cytokines.

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