100mL
2~8℃, keep away from light
12 months
Liquid
1.1 Add 1mL of 293 supplementation medium at 24h and 72h after transfection (the total amount of addition is 10% of the initial transfection volume), and maintain the sugar content during the culture period is not less than 2g/L;
1.2 When the cell activity rate drops to about 70% (or according to protein accumulation), harvest the cell supernatant or cell precipitation, and determine the expression of the product.
2.1 After cell transfection, the volume and times of additions of the supplementation medium can be adjusted according to the change of sugar content during cell culture;
2.2 In the production of HEK293 engineering cell lines, the supplementation medium can also be used to increase the components required for cell growth ,so as to improve the growth density of cells and the expression level of target proteins.
AMMS® HEK293 supplementation medium, which does not contain any animal origin ingredients and L-glutamine, is specially used for HEK293 cell transfection or production process, Support batch cell culture with supplementary materials to meet the requirements of customized culture media..This product is only used for scientific research, it can not be used for clinical, food, etc.
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