038124

human

2×10⁸beads/mL

< 0.5EU/mL

棕色悬浊液体

2~8℃

24 months

1. After cell expansion, the cell suspension in the culture vessel should be gently blown periodically to allow adequate dispersion of the magnetic beads and cells.

2. During the culture process, the cells could be counted every 2 to 3 days, and the magnetic beads should be removed before cell counting. The magnetic beads removal process: the cells in the culture vessels were evenly suspended and sampled into the centrifuge tube, placed on the magnet, left for 1 min, and then transfer the supernatant to a new centrifuge tube.

3. After the cells were cultured for a suitable number of days, the cells needed to be harvested after removing the magnetic beads.

4. The purity, phenotype and activation state of T cells could be detected by flow cytometry during cell culture.(e.g. after 48 hours of cell culture, test the percentage of CD69⁺CD25⁺ on the cell membrane after removing the beads to evaluate the activation effect of magnetic beads.)